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Beads of Sephacryl S200-S1000, Sephadex G-1-00, Sepharose 2B-6B and Sephadex G-50 can be used for size exclusion chromatography

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After propagation of USCs in 50% CM over several passages and further culturing in 50% CM over 5 d, all cells stained positive for the epithelial cell marker E-cadherin and the basal urothelial cell marker keratin 5 (K5) but lacked Upk3a expression (Extended Data Fig