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We investigated the sperm DNA integrity using acridine Orange (AO) assay and Chromomycin staining (CMA3)

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Research in South American tobacco fields shows that organic amendments and biochar: Increase populations of beneficial nitrogen-fixing and phosphorus-solubilizing microorganisms Enhance nutrient cycling in the rhizosphere Reduce nutrient losses while maintaining steady nutrient supply to plants This soilmicrobeplant interaction is a fundamental mechanism enabling reduced fertilizer inputs without yield penalties

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