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Live-cell microscopy RAW 264.7 macrophages were seeded in Nunc 8-well Lab-Tek chambered coverglass (ThermoFisher) at 23 10 5 cells per well in 500 l complete minimum essential medium (MEM, Gibco) supplemented with 10% (v/v) heat-inactivated FBS and 2 mM L-glutamine at 37 C with 5% CO 2 for 16 h

These findings align with previous research demonstrating the therapeutic potential of natural products like Cinnamaldehyde, Sarsasapogenin-AA13, Ginkgo biloba extracts, and active components of Andrographis paniculata in targeting these pathways for AD treatment [49, 89,90,91,92]

Mini Rev Med Chem 18:10951109

Shi S, Liang D, Bao M, Xie Y, Xu W, Wang L, Wang Z, Qiao Z (2016) Gx-50 inhibits neuroinflammation via 7 nAChR activation of the JAK2/STAT3 and PI3K/AKT pathways