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If stubborn particles remain, let the vial sit for a few minutes and roll again

The 10 structural models with the lowest associated energy from 100 calculated structural models were used to prepare figures using the PyMOL Molecular Graphics System, Version 2.4 (Schrdinger, LLC)

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At a determined period of time (e.g., 6 hours, 12 hours, 1 day, 2 days, 3 days, 4 days, 5 days, 6 days, 7 days, and 14 days for testing the pharmacokinetics of a formulation or composition over a 1 or 2 week period), the test animal is euthanized and the inner ear removed and tested for the presence of the otic agent