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A user then inhales the smoke through a tube and mouthpiece

The control experiment, consisting of untreated CA substrate, was included to ensure a clear comparison between the control and pretreated samples

Abbreviations: AM (alveolar macrophage), BAL (bronchoalveolar lavage), DC (dendritic cell), e-cig (e-cigarette), e-cig vaped extract (EVE), gen (generation), h (hour), min (minute), NK (natural killer), PG (propylene glycol), THC (tetrahydrocannabinol), VG (vegetable glycerin) Dendritic cells (DCs) are professional antigen presenting cells and thus play a critical role in bridging innate and adaptive immunity with the goal of providing immune surveillance, maintaining immune tolerance, and activating T-cells and inflammatory responses in the setting of infections and vaccinations