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Bulk RNA-sequencing Human CD8 + T cells were isolated from PBMCs and activated and cultured with or without ML226 for 11 days as previously described

Elemental analysis was performed using inductively coupled plasmaoptical emission spectroscopy as described previously ( Ferric Chelate Reductase Assay Ferric chelate reductase assays were performed as previously described ( atgrxs17 KO, and AtGRXS17 RNAi seeds were germinated and grown on MS medium for 10 days at 22C under 16 h light and 8 h dark

Quantitation of erythropoietin-producing cells in kidneys of mice by in situ hybridization: correlation with hematocrit, renal erythropoietin mRNA, and serum erythropoietin concentration

Cellular senescence is known to increase proinflammatory cytokine release linked to aging