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After blocking with 10% normal goat serum in PBS at room temperature for 1h, the cells were incubated with mouse anti-PPAR- (primary antibody, 1:1000 dilution) at 4C for 12h, followed by incubation with FITC-conjugated goat anti-mouse IgG (second antibody, 1:3000 dilution) at 37C for 60min

In order to evaluate the effects of each antioxidant, primary skin fibroblasts from a middle-aged normal individual and an HGPS patient were treated for 4 weeks