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C., Karkos, C

It is important to note that serum Se (S-Se) does not directly reflect the concentration of Se in tissues, and even normal S-Se content does not exclude the possibility of Se deficiency in the thyroid (119, 120)

The remaining 23 mL of each culture was quickly filtered under vacuum (Air Cadet vacuum/pressure station, Thermo), immediately washed with cold saline solution (9.5 g L 1 NaCl at 12 C) and quenched in cold methanol/water (1:1 v/v) at 30 C as described by Smart et al