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All lanes: Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (ab125066) at 1/1000 dilution Lane 1: Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 g Lane 2: GPX4 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 g Lane 2: Western blot - Human GPX4 knockout HeLa cell line (ab262509) Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 g Predicted band size: 22 kDa Observed band size: 20 kDa false WB Lab Western blot - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066) Blocking/Diluting buffer and concentration 5% NFDM/TBST

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Gut microbiota in ulcerative colitis: insights on pathogenesis and treatment