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A 10 ml sample of each culture was harvested by centrifugation at 5000 g for 10 min and resuspended in 4 ml of lysis buffer (50 mM NaH 2 PO 4 /Na 2 HPO 4 , 0.3 M NaCl, 20 mM imidazole, pH 8.0), then lysed by sonication on ice

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accidentally using opioids instead of stimulants in groups where people are using multiple drugs smoking stimulants with a pipe that had previously been used to smoke fentanyl One participant explained how they had overdosed as a result of sharing supplies: the first time I [overdosed] it was with a group of friends andI didnt realize someone was smoking fentanyl out of their pipe, and just went down... Responding to smoking-related overdose Nearly half of study participants had responded to a smoking-related overdose