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All subjects were asked to abstain from smoking, coffee, and other foods or beverages containing caffeine (e.g., tea, cola, cacao, guarana) for a wash-out period of at least 12 hours before examinations

There were no significant changes in lymphocytes, although CC-LR/TLR4KO showed a significant decrease in macrophages, and CC-LR/TLR9KO showed a significant increase ( Figure 3A )