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We hypothesize that (i) the slow inhibition observed in the biochemical assay with the recombinant protein with some of the compounds is due to their preferential binding to a slowly populated conformer of the NADP + -TGR(H) complex and (ii) this conformer is already populated in the worm cell interior accounting for the more rapid inhibition of TGR observed in ex vivo worms with the slow inhibitors

The moderation analysis revealed that pooled effect sizes for reduced cortical tCho were greater across studies with better metabolite measurement quality, further validating the primary finding

GSSG eventually generates GSH after cutaneous absorption