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Im not a chemist, but Ive been a Pure Life coffee enema user since 2021, and I know the product works as evidenced by the fact that I had a rash on my inner thighs which indicated that my liver was not detoxing properly, and as long as I use the coffee enemas regularly I have no skin rash issues, and my stools are a healthy color, which indicates adequate bile production

These anaerobes produce metabolites, including short-chain fatty acids, polyamines, ammonia, and volatile amines, that can irritate the epithelium, damage DNA, and cause oxidative stress

Small mobilizable multi-purpose cloning vectors derived from the Escherichia coli plasmids pK18 and pK19: selection of defined deletions in the chromosome of Corynebacterium glutamicum

1996;154(4 Pt 1):1055-1060