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Most studies merely confirm the association between Clostridium /its metabolites and classical signaling pathways, without identifying the direct targets of action on target cells

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After different groups of HUVECs are treated accordingly, they are seeded into 24-well plates pre-coated with 150 L/well growth factor-reduced Matrigel (Corning, catalog number 354234) and incubated at 37 C in a cell culture incubator for 12 h