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Many studies have found that carotenoids can affect the expression of cell cycle-related proteins, such as increasing the levels of the cell cycle inhibitors p21Cip1 and p27Kip1, while inhibiting the activity of Cyclin and its related kinases, thereby inducing cell cycle arrest in cancer cells ( In addition to inhibiting proliferation, carotenoids affect the cellular DNA damage response pathway, enhancing p53-mediated gene monitoring and repair

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After the amplification, a melting peak analysis with a temperature gradient from 72 C to 95 C was performed