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In another study, chlorophyllin treatment has been reported to cause cell cycle arrest in HCT116 colon cancer cells through the increased expression of E2F1 and E2F4 transcription factors inhibiting the G1/S checkpoint step, which is an important cell cycle checkpoint (Chimploy et al

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To ensure consistent peptide concentrations across samples, the digested peptides were quantified using the Pierce Quantitative Colorimetric Peptide Assay (#23275, Thermo Fisher Scientific), a modified BCA assay