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During the acquisition phase, the platform was placed 1 cm above the water level so that the rats can quickly identify the platform and sit on it when forced to explore the tank for 2 min

In a previous study, researchers established a HepG2 cells model containing glucose (30 mM) and palmitic acid (0.1 mM)

The activity of cellular tyrosinase was then visualized in the gel as dark melanin-containing bands and quantified in terms of color intensity using the LabWorks program (UVP, Upland, CA, USA) for the percentage mammalian tyrosinase activity with reference to control (without treatment)

This corrected matrix was then analysed using DESeq2 to calculate the adjusted P value in the covariate model