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The cells were treated with 25uM FOXO4 peptide 8h after starting the measurements

GSNO maintained cell-membrane permeability, increased levels of chloroplast membrane lipids (monoglycosyldiacylglycerol, phosphatidylglycerol, and digalactosyldiacylglycerol), improved -3 FAD enzyme activity, enhanced ascorbateglutathione cycling, and promoted BoFAD8 transcription, thus promoting 18/16:3-triene fatty acid accumulation