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Antibodies to kidney injury molecule KIM-1, 4-HNE 4-hydroxynonenal, GPX4, xCT, Ferritin, FPN, and -actin were purchased from Abcam (Cambridge, United States)
Based on the here-presented biochemical, structural and computational evidence, we propose that arGSTs utilize a GSH-dependent Brnsted base mechanism for conversion of the semi-stable LDOX product flavan-3,3,4-triol ( 4 ) or flavan-3-on-4-ol ( 7 ) into flav-2-en-3,4-diol ( 8 -B4), the 4-hydration species of cyanidin ( 8 ) (Extended Data Fig
Purification of the protein was done with a HISTrap column connected to the kta_prime_plus chromatography
Glutathione peroxidase catalysis occurs in the presence of reduced glutathione, a tripeptide, in a paired reaction involving glutathione reductase, which regenerates reduced glutathione from oxidized glutathione (Handy and Loscalzo 2022)
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