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Residual Solvents Determined in accordance with the general method for the determination of residual solvents (General Chapters 0861 Method 2)

In some embodiments, dyes that are compatible with the otic compositions described herein include Evans blue (e.g., 0.5% of the total weight of an otic formulation), Methylene blue (e.g., 1% of the total weight of an otic formulation), Isosulfan blue (e.g., 1% of the total weight of an otic formulation), Trypan blue (e.g., 0.15% of the total weight of an otic formulation), and/or indocyanine green (e.g., 25mg/vial)

Importantly, preclinical studies showed that weight reduction occurred particularly in abdominal adipose tissue, the visceral fat depot most strongly associated with metabolic health risks