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Bound monovalent HA Fc fusions or anti-NIP IgG1 variants were detected by an anti-human IgG Fc specific AP-conjugated antibody from goat (Sigma-Aldrich, A9544) (diluted 1:5000 in PBS/T/S) and visualized by addition of p-nitrophenylphosphate (Merck) (10 g/mL in diethanolamine buffer)

Horm Metab Res 50(12):837839 [DOI] [PubMed] [Google Scholar] Bao Y, Kim D, Cho YH, Ku CR, Yoon JS, Lee EJ (2023) Cre-loxp system-based mouse model for investigating Graves disease and associated orbitopathy

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People who should not take lipotropic injections include those who are pregnant or breastfeeding, individuals with a known allergy to any of the injection ingredients (particularly sulfa-related sensitivities due to methionine), people with severe liver or kidney disease, and those taking certain medications that may interact with the supplement ingredients