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Detailed biophysical studies (X-ray crystallography, EPR, NMR) elucidated that in GHK-Cu, the Cu(II) ion is coordinated by the imidazole side chain nitrogen of histidine, the alpha-amino nitrogen of glycine, and the deprotonated amide nitrogen of the glycine-histidine bond

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This age-related decline correlates with diminished wound healing capacity, reduced collagen synthesis, and compromised tissue regenerationobservations that sparked decades of research into GHK-Cu's role in aging and tissue maintenance