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Metal catalyzed nucleophilic substitution: Here, instead of a proton donor, a metal, which could be Zn 2+ , binds to the cysteine sulfur giving it a negative charge so it is equivalent to a thiolate

Finally, we detected apoptosis, proliferation, and migration in various treatment groups

Glutathione 250mg alone, however, was not significantly superior to placebo.52 Another recent RCT (n=46) reported decreased skin pigmentation following an 8-week regimen of 600mg of glutathione, 50mg of -lipoic acid, and 4mg of zinc.53 Even better results were observed for oral glutathione combined with topical 2% glutathione