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Finally, 500 ng total RNA was used as a template for the cDNA reaction, which was synthesized using the iScriptcDNA Synthesis Kit (Bio-Rad, Veenendaal, the Netherlands)

Meanwhile ICP27 can act as a sequence-dependent activator of mRNA 3 processing for viral transcripts

This hyper-proliferative state drains the body's metabolic energy and drives the profound fatigue seen in these conditions