Reconstituted solutions prepared with an appropriate laboratory diluent are generally kept refrigerated at 2-8C and used within the window validated by the research protocol
Hayashi Y, Correa AM, Hofstetter WL, Vaporciyan AA, Mehran RJ, Rice DC, Suzuki A, Lee JH, Bhutani MS, Welsh J, Lin SH, Maru DM, Swisher SG , Ajani JA
None of the studies cited above controlled for where the women or animals were during the menstrual (or estrus) cycle when the measurements were made
Ideally, the injection is administered into a fatty layer of skin such as the back of the arm, abdomen, or flank/lumbar region (side of the body between the rib cage and iliac bone of the hip)