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Fasman (19252003), demonstrated how CD spectra could be computationally analyzed to estimate the secondary structural composition of proteins (Greenfield and Fasman, 1969)

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2.11 Separation and extraction of nuclear and cytoplasmic proteins In this experiment, nuclear and cytoplasmic fractionation was performed using the Nuclear and Cytoplasmic Protein Extraction Kit (Beyotime, P0027) following the manufacturers instructions

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