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By using homogenizer (Tri-R Stir-R homogenizer, Tri-R Instruments, Inc., Rockville Centre, NY), every one gram of lung tissue was homogenized in 5 ml of phosphate buffer saline (PBS) which prepared by dissolving 0.2 g KCl, 8.01 g NaCl, 0.27 g KH 2 PO 4 and 1.78 g Na 2 HPO 4 .2H 2 O in 1 litter of distilled water and adjust the PH at 7.4 then this homogenate was centrifuged for 15 min at 4000 g

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