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Subsequently, each system underwent independent simulations lasting 500 ns each (totaling 1.5 s per system) at 298 K, employing the Langevin thermostat with a time step of 0.2 fs (Supplementary Fig

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They were then transfected with 6 g of the pMXs-3XHA-EGFP-OMP25 (Addgene, 83356) plasmid in the presence of polyethylenimine