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After blocking with Superblock T-20 (Thermo Scientific, Rockford, IL) membranes were incubated for overnight with primary antibody against GLUT1 for astrocytes and GLUT3 for neurons (11000, Abcam, Cambridge, MA) at 4C followed by 1 hr incubation with secondary antibodies conjugated with horse-radish peroxidase

Both GLS silencing and GLS2 overexpression synergize with oxidative stress against proliferation of glioma cells

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Ruiz, S., Pergola, P