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Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole

Importantly, recent studies indicate that deficiency in Prx6-dependent selenium delivery can significantly decrease GPX4 protein level, with a corresponding reduction in ferroptosis sensitivity of similar magnitude

Hormone-sensitive lipase (HSL) in adipocytes is activated to produce the lipolytic effect, which is then enhanced by indirect processes such as secretion of growth hormone, cortisol, and adrenaline [39]